datura stramonium agglutinin (Vector Laboratories)

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Vector Laboratories datura stramonium agglutinin
Datura Stramonium Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/L-1180-5/Unconjugated+Datura+Stramonium+Lectin+(DSL)/pmc11462596-45-45-80
Average 93 stars, based on 26 article reviews

datura stramonium agglutinin - by Bioz Stars, 2026-11

93/100 stars

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Incubation:

Article Title: Sex-Dependent Differences in Blood-Urine Barrier Are Subtle but Significant in Healthy and Chronically Inflamed Mouse Bladders.
Article Snippet: .. Subsequently, the sections were incubated with various FITC-conjugated lectins (Concavalin A (ConA) at 1:200, Datura stramonium lectin (DSL) at 1:100, Jacalin at 1:400, Wheat Germ agglutinin (WGA) at 1:800, all from Vector Labs, Burlingame, CA, USA) in PBS for 1 h at RT. .. Nuclei were visualized using Höechst staining (Thermo Fisher Scientific, Carlsbad, SA, USA) for 15 min at RT, and sections were mounted in Vectashield (Vector Labs, Burlingame, CA, USA).

Article Title: Sex-Dependent Differences in Blood–Urine Barrier Are Subtle but Significant in Healthy and Chronically Inflamed Mouse Bladders
Article Snippet: .. Subsequently, the sections were incubated with various FITC-conjugated lectins (Concavalin A (ConA) at 1:200, Datura stramonium lectin (DSL) at 1:100, Jacalin at 1:400, Wheat Germ agglutinin (WGA) at 1:800, all from Vector Labs, Burlingame, CA, USA) in PBS for 1 h at RT. .. Nuclei were visualized using Höechst staining (Thermo Fisher Scientific, Carlsbad, SA, USA) for 15 min at RT, and sections were mounted in Vectashield (Vector Labs, Burlingame, CA, USA).

Whole Genome Amplification:

Article Title: Preoperative Serum Glycan Levels Reflect Progression of Patients With Hepatocellular Carcinoma
Article Snippet: .. Lectins, including Aleuria aurantia lectin (AAL, L‐1390‐2), Lotus tetragonolobus lectin (LTL, L‐1320‐5), Ulex europaeus agglutinin (UEA‐I, L‐1060‐2), Lens culinaris agglutinin (LCA, L‐1040‐10), Jacalin (JAC, L‐1150‐25), Ricinus communis agglutinin I (RCA‐I, L‐1080‐10), Vicia villosa lectin (VVL, L‐1230‐5), Concanavalin A (ConA, L‐1000‐500), Narcissus pseudonarcissus lectin (NPL, L‐1370‐5), Datura stramonium agglutinin (DSA, L‐1180‐5), Wheat germ agglutinin (WGA, L‐1020‐10), PNA (L‐1070‐5), Maackia amurensis lectin I (MAL‐I, L‐1310‐5), Sambucus nigra agglutinin (SNA, L‐1300‐5), Phaseolus vulgaris Leucoagglutinin (PHA‐L, L‐1110‐5), and biotinylated NPL (B‐1375‐2) were purchased from Vector Laboratories Inc. (CA, USA). .. Dimethyl sulfoxide (DMSO, 41640), bovine albumin (BSA, B2064), proteo Prep immunoaffinity albumin and IgG depletion kit (PROTIA), phosphate‐buffered saline (PBS, P3813), and 2‐[4‐(2‐hydroxyethyl) piperazin‐1‐yl] ethanesulfonic acid (HEPES, V900479) were commercially obtained from Sigma (MO, USA).

Article Title: Toward acellular xenogeneic heart valve prostheses: Histological and biomechanical characterization of decellularized and enzymatically deglycosylated porcine pulmonary heart valve matrices.
Article Snippet: .. Effects of the various treatments on carbohydrates removal were investigated using lectins, for example, isolectin B4 (IL-B4/GSL-IB4) (1:100, #B-1205, Vector Laboratories Inc), specific for α-galactose residues; wheat germ agglutinin (WGA) (1:200, #RL-1022, Vector Laboratories Inc); datura stramonium lectin (DSL) (1:200, #B-1185, Vector Laboratories Inc); and ricinus communis agglutinin I (RCA-I) (1:200, #RL-1082, Vector Laboratories Inc). .. IL-B4- and DSL-stained sections were additionally stained with avidin-linked Texas Red (1:500, #A-2006; Vector Laboratories Inc).

Staining:

Article Title: N-glycosylation engineering in chimeric antigen receptor T cells enhances anti-tumor activity
Article Snippet: Transduced cells were detected by eGFP expression or by an anti-VHH antibody (Genscript) directed against the nanobody constituting the extracellular domain of the CAR and analyzed by flow cytometry. .. For the evaluation of the poly-LacNAc content on the cell surface, we used the lectin from Datura stramonium at a staining concentration of 10 μg/ mL (Biotinylated DSL, Vector laboratories, B-1185-2). ..

Article Title: ‘Fetal side’ of the placenta: anatomical mis-annotation of carbon particle ‘transfer’ across the human placenta
Article Snippet: .. Paraformaldehyde-fixed term human placental villi were stained with FITC-Aleuria Aurantia Lectin to visualise blood vessels, Biotin Datura Stramonium Lectin with Streptavidin 680 to visualise the trophoblast and Rhodamine-labelled Pisum sativum Agglutinin which binds the stroma (all Vector Laboratories), and imaged by confocal microscopy. ..

Article Title: Toward acellular xenogeneic heart valve prostheses: Histological and biomechanical characterization of decellularized and enzymatically deglycosylated porcine pulmonary heart valve matrices.
Article Snippet: Effects of the various treatments on carbohydrates removal were investigated using lectins, for example, isolectin B4 (IL-B4/GSL-IB4) (1:100, #B-1205, Vector Laboratories Inc), specific for α-galactose residues; wheat germ agglutinin (WGA) (1:200, #RL-1022, Vector Laboratories Inc); datura stramonium lectin (DSL) (1:200, #B-1185, Vector Laboratories Inc); and ricinus communis agglutinin I (RCA-I) (1:200, #RL-1082, Vector Laboratories Inc). .. IL-B4- and DSL-stained sections were additionally stained with avidin-linked Texas Red (1:500, #A-2006; Vector Laboratories Inc). ..

Concentration Assay:

Saline:

Article Title: Altered profile of glycosylated proteins in serum samples obtained from patients with Hashimoto′s thyroiditis following depletion of highly abundant proteins
Article Snippet: The separated proteins were transferred to PVDF membranes (Millipore, Billerica, MA, USA) while the duplicate gels were stained with Coomassie brilliant blue. .. The membranes were blocked with 1% BSA in phosphate buffered saline with 0.1% Tween 20 (PBS-T) at room temperature for 1 h and probed with biotinylated lectins, including Vicia villosa lectin (VVA), Maackia amurensis lectin (MAA), Solanum tuberosum lectin (STL), Sambucus nigra I lectin (SNA-I) and Datura stramonium lectin (DSA) (1:400; Vector Laboratories Inc., US) at 4°C overnight in the dark. .. After three washes with PBS-T, the membranes were incubated with HRP-conjugated streptavidin (Yeasen, Shanghai, China).

Confocal Microscopy:

Avidin-Biotin Assay:

Article Title: Toward acellular xenogeneic heart valve prostheses: Histological and biomechanical characterization of decellularized and enzymatically deglycosylated porcine pulmonary heart valve matrices.
Article Snippet: Effects of the various treatments on carbohydrates removal were investigated using lectins, for example, isolectin B4 (IL-B4/GSL-IB4) (1:100, #B-1205, Vector Laboratories Inc), specific for α-galactose residues; wheat germ agglutinin (WGA) (1:200, #RL-1022, Vector Laboratories Inc); datura stramonium lectin (DSL) (1:200, #B-1185, Vector Laboratories Inc); and ricinus communis agglutinin I (RCA-I) (1:200, #RL-1082, Vector Laboratories Inc). .. IL-B4- and DSL-stained sections were additionally stained with avidin-linked Texas Red (1:500, #A-2006; Vector Laboratories Inc). ..